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Maintaining a near physiologic environment in vitro is vital for the growth of embryos. Advances in the development of embryo culture systems including media, equipment and techniques have improved embryo viability significantly resulting in better pregnancy and live birth rates.
Media are vital ingredients as the gametes or embryos develop within them. Media may be of different types such as simple salt solutions with added nutrients or complex fortified media, simple optimized media or sequential media. Media use is governed by the requirements of the growing embryo and changing needs from pronucleate to cleavage stage to blastocyst stage. The chances of culture media induced trauma/damage to the embryo should be minimal. Birth defects attributable to improper media composition have been described. |
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